Fluorescence Microscopy Techniques for Microbiologists

Workshop

* places are limited

Registration includes

• Lecture notes & recommended protocols

• Afternoon tea

How to Register

• Register online
  (NB. You can register for workshop without registering for the ASM 2010 Sydney Annual Conference)

• OR: if you have already registered for the Annual Conference, then simply log in to the Edit Environment using your Access Key and register for the workshop.

Workshop Convenors

Associate Professor Cynthia Whitchurch NHMRC Senior Research Fellow, Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney NSW

Dr Lynne Turnbull Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney NSW

Hosted By

Molecular Microbiology Special Interest Group, ASM

Overview

This workshop will introduce the fundamentals of fluorescence microscopy techniques specifically aimed at the needs of microbiologists.

Lectures will include a brief introduction to the major fluorescence microscopy techniques including widefield, deconvolution, confocal and super-resolution. We will discuss the basics of sample preparation for microbiologists including the use and choice of fluorophores.

Techniques for live imaging of microorganisms and their interactions with host cells and the challenges involved will be discussed. We will also cover recent advances in super-resolution microscopy that have opened new avenues for the exploration of the cell biology of microorganisms and host cells.

The workshop will conclude with tours of the UTS Microbial Imaging Facility and demonstrations of live imaging, confocal imaging and super-resolution 3D-SIM OMX imaging systems.

Workshop participants will also have the opportunity for limited imaging of their own samples using the UTS Microbial Imaging Facility equipment including the DeltaVision OMX 3D-SIM super-resolution microscope. Sample preparation and delivery must be co-ordinated with convenors prior to the workshop.

Preliminary Program  (may be subject to change)

Detailed Directions

University of Technology, Sydney (UTS), City Campus, Building 4

Corner Harris and Thomas Streets, Ultimo, NSW 2007.

Enter the building from Harris Street entrance (cnr Thomas St) and go up the stairs to registration desk on level 2. Delegates will then be advised the direction of their lecture room. No on-site parking available at UTS - Only street parking or at nearby carparks.

Facilities:

Excellent in each room and lab. Desk top microphone built into lectern, data projector, large screens, DVD, CD, document camera with zoom facility. PC compatible (no Macs), can plug in laptop. Cannot save to desktop, therefore, for smooth transition between speakers best to save all presentations on one memory stick. 

Directions to get to UTS, Building 4:

Tell taxi drivers to take you to the ABC Building on Harris St. Most know where that is. Cross the road at the lights and you are at the Science Faculty (UTS, Building 4).

From Darling Harbour, walk through to Tumbalong Park and through Chinatown to Ultimo Rd until you reach Harris St (ask for a map at your hotel). Turn left and you will see the shiny silver Science Faculty building on the corner ahead of you. It’s an 8-10 minute walk. Or catch a cab ($7-10 depending on traffic)!

The UTS City campus, Broadway, is close to Central railway station. After alighting at Central Station take the exits to Railway Square/George Street (via the "Devonshire Tunnel", a pedestrian tunnel). Turn left at Railway Square (towards the big brown tower that says UTS!) and walk one block to Harris St, cross at the lights  and turn right at the Co-op bookshop (UTS Broadway campus). Walk down the road until you get to Thomas St. You should be outside the Science Faculty building (Building 4) with the ABC building across the road.

If you catch any bus that goes to Railway Square (George St), turn towards the big brown tower that says UTS and walk one block to Harris St, cross at the lights  and turn right at the Co-op bookshop (UTS Broadway campus). Walk down the road until you get to Thomas St. You should be outside the Science Faculty building (Building 4, shiny and silver) with the ABC building across the road.

It is easiest to enter Building 4 from the corner of Thomas and Harris Sts and make your way to the lifts or use the stairs directly in front of you as registration is at the top of these stairs on Level 2. Do not use the entrance and lifts that are halfway up the building on Harris St as these are pin-coded and you will get stuck on Level 4.

Below is a link to the campus map:

http://www.uts.edu.au/about/mapsdirections/citymap.html

The registration desk and workshop rooms are on Level 2; at the top of the stairs in the main entrance.